Noninvasive genetic sampling of endangered muriqui (Primates, Atelidae): efficiency of fecal DNA extraction

Genetics and Molecular Biology

Paulo B. Chaves²  Marcela F. Paes²  Sérgio L. Mendes¹  Karen B. Strier¹  Iúri D. Louro¹  Valéria Fagundes² 

[1] ,Universidade Federal do Espírito Santo Departamento de Ciências Biológicas Laboratório de Genética AnimalVitória ES ,Brazil

关键词: Brachyteles;    conservation genetics;    endangered species;    fecal DNA;    noninvasive sampling;   

DOI  :  10.1590/S1415-47572006000400028

来源: SciELO

【 摘 要 】

The muriqui (Brachyteles) is one of the most endangered primates in the world, however little is known about the viability of the remaining populations. We evaluated the technique of extracting DNA from wild muriqui feces for PCR applications. In order to determine the effect of the DNA in subsequent amplifications, we analyzed five different extracts. The importance of the recommended BSA and the HotStarTaq DNA polymerase was tested. The minimal conditions to successfully amplify highly degraded fecal DNA were determined, showing that the recommended reagents are not required. We envision that this method may be useful in further conservation management studies.

【 授权许可】

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