Isolation and sequencing of seven Sox genes from the lacertid lizard Eremias breuchleyi

Genetics and Molecular Biology

Li Jie¹  Ping-ping Zheng¹  Jiao-lian Song¹  Jin-long Rui¹  Liu-wang Nie¹ 

[1] ,Anhui Normal University Life Science College Wuhu Anhui province ,China

关键词: Eremias breuchleyi;    PCR;    sequence analysis;    Sox genes;    SSCP;   

DOI  :  10.1590/S1415-47572006000300031

来源: SciELO

【 摘 要 】

The Sox family of genes shares a high sequence similarity with the HMG box region of the human Y chromosomal gene, SRY. We used highly degenerate primers to clone and sequence seven Eremias breuchleyi Sox genes (EbSox2, EbSox3, EbSox4, EbSox11, EbSox12, EbSox14 and EbSox21). A database search for the cloned sequences revealed the following percentage identity with the homologous human SOX genes: EbSox2 = 96%, EbSox3 = 88%, EbSox4 = 94%, EbSox11 = 99%, EbSox12 = 96%, EbSox14 = 98%, EbSox21 = 97%. Cluster analysis indicates that they seem to belong to group B and group C of Sox gene family, respectively.

【 授权许可】

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