Mitochondrial DNA D-loop sequence variation among 5 maternal lines of the Zemaitukai horse breed

Genetics and Molecular Biology

E. Gus Cothran²  Rytis Juras²  Vale Macijauskiene¹ 

[1] ,University of Kentucky Department of Veterinary Science Lexington KY ,USA

关键词: D-loop;    Equus caballus;    phylogeny;    polymorphism;   

DOI  :  10.1590/S1415-47572005000500006

来源: SciELO

【 摘 要 】

Genetic variation in Zemaitukai horses was investigated using mitochondrial DNA (mtDNA) sequencing. The study was performed on 421 bp of the mitochondrial DNA control region, which is known to be more variable than other sections of the mitochondrial genome. Samples from each of the remaining maternal family lines of Zemaitukai horses and three random samples for other Lithuanian (Lithuanian Heavy Draught, Zemaitukai large type) and ten European horse breeds were sequenced. Five distinct haplotypes were obtained for the five Zemaitukai maternal families supporting the pedigree data. The minimal difference between two different sequence haplotypes was 6 and the maximal 11 nucleotides in Zemaitukai horse breed. A total of 20 nucleotide differences compared to the reference sequence were found in Lithuanian horse breeds. Genetic cluster analysis did not shown any clear pattern of relationship among breeds of different type.

【 授权许可】

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