Boron transport in Eucalyptus. 2. Identification in silico of a putative boron transporter for xylem loading in eucalypt

Genetics and Molecular Biology

Douglas Silva Domingues²  Susi Meire Maximino Leite²  Ana Paula Cazerta Farro²  Virgínia Elias Coscrato²  Edson Seizo Mori¹  Edson Luiz Furtado¹  Carlos Frederico Wilcken¹  Edivaldo Domingues Velini¹  Iraë Amaral Guerrini¹  Ivan Godoy Maia²  Celso Luis Marino² 

[1] ,Universidade Estadual Paulista Instituto de Biociências Departamento de GenéticaBotucatu SP ,Brazil

关键词: Eucalyptus;    boron;    xylem loading;    Expressed Sequence Tag (EST);    BOR1;   

DOI  :  10.1590/S1415-47572005000400017

来源: SciELO

【 摘 要 】

Boron (B) is a low mobility plant micronutrient whose molecular mechanisms of absorption and translocation are still controversial. Many factors are involved in tolerance to Boron excess or deficiency. Recently, the first protein linked to boron transport in biological systems, BOR1, was characterized in Arabidopsis thaliana. This protein is involved in boron xylem loading and is similar to bicarbonate transporters found in animals. There are indications that BOR1 is a member of a conserved protein family in plants. In this work, FORESTS database was used to identify sequences similar to this protein family, looking for a probable BOR1 homolog in eucalypt. We found five consensus sequences similar to BOR1; three of them were then used in multiple alignment analysis. Based on amino acid similarity and in silico expression patterns, a consensus sequence was identified as a candidate BOR1 homolog, helping deeper experimental assays that could identify the function of this protein family in Eucalyptus.

【 授权许可】

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