Eucalyptus ESTs corresponding to the protoporphyrinogen IX oxidase enzyme related to the synthesis of heme, chlorophyll, and to the action of herbicides

Genetics and Molecular Biology

Edivaldo Domingues Velini²  Maria Lúcia Bueno Trindade²  Elza Alves²  Ana Catarina Catâneo¹  Celso Luis Marino¹  Ivan De Godoy Maia¹  Edson Seizo Mori¹  Edson Luiz Furtado¹  Iraê Amaral Guerrini¹  Carlos Frederico Wilcken¹ 

[1] ,Universidade Estadual Paulista 'Júlio de Mesquita Filho' Faculdade de Ciências Agrárias Fazenda Experimental LageadoBotucatu SP ,Brazil

关键词: Eucalyptus;    protoporphyrinogen IX oxidase;    catalase;    peroxidase;    herbicide;   

DOI  :  10.1590/S1415-47572005000400009

来源: SciELO

【 摘 要 】

This work was aimed at locating Eucalyptus ESTs corresponding to the PROTOX or PPO enzyme (Protoporphyrinogen IX oxidase, E.C. 1.3.3.4) directly related to resistance to herbicides that promote oxidative stress, changing the functionality of this enzyme. PROTOX, which is the site of action of diphenyl-ether (oxyfluorfen, lactofen, fomesafen), oxadiazole (oxadiazon and oxadiargyl), and aryl triazolinone (sulfentrazone and carfentrazone) herbicides, acts on the synthesis route of porphyrins which is associated with the production of chlorophyll a, catalases, and peroxidases. One cluster and one single read were located, with e-values better than e-70, associated to PROTOX. The alignment results between amino acid sequences indicated that this enzyme is adequately represented in the ESTs database of the FORESTs project.

【 授权许可】

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