Analysis by RAPD of the genetic structure of Astyanax altiparanae (Pisces, Characiformes) in reservoirs on the Paranapanema River, Brazil

Genetics and Molecular Biology

Maria Sueli Papa Leuzzi²  Fernanda Simões De Almeida²  Mário Luís Orsi¹  Leda Maria Koelblinger Sodré² 

[1] ,Universidade Estadual de Londrina Departamento de Biologia Geral Laboratório de Marcadores Moleculares em Peixes e Ecologia de AbelhasLondrina PR ,Brazil

关键词: Astyanax altiparanae;    RAPD;    genetic structure;    genetic conservation;    fish;   

DOI  :  10.1590/S1415-47572004000300009

来源: SciELO

【 摘 要 】

In this study, the RAPD technique was used to analyze the genetic structure of populations of the fish Astyanax altiparanae (Characidae, Tetragonopterinae) living in the lower, middle and upper Paranapanema River, Brazil. The aim was to assess this structure regarding fish handling and conservation programs. The genetic variability (P) was found to be 42.64%, 75% and 75% in the low, middle and upper reaches, respectively. The dendrogram of genetic similarity, obtained by comparative analysis of the sets of samples from the three sites, showed the formation of three clusters. All of the genetic parameters used indicate that the population in the lower Paranapanema is genetically different from those in the middle and upper sections. The theta P test shows that the low Paranapanema is highly differentiated from the middle (0.2813) and upper (0.2912) Paranapanema, while the differentiation between the last two is moderate (0.0895). The data obtained in the present work suggest that recolonization and conservation studies should not be focused on the species A. altiparanae as such, but on the conservation units, because they are the genetically differentiated populations.

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