A rapid alternative technique for obtaining silver-positive patterns in chromosomes

Genetics and Molecular Biology

Karine Frehner Kavalco¹  Rubens Pazza¹ 

[1] ,Universidade Federal de São Carlos Departamento de Genética e Evolução São Carlos SP ,Brazil

关键词: silver staining;    Ag-NOR;    microwaves;    chromosome banding;   

DOI  :  10.1590/S1415-47572004000200012

来源: SciELO

【 摘 要 】

Silver nitrate chromosome staining to evidence nucleolar organizer regions (NORs) is a widely adopted methodology. The aim of the present work was to improve this technique, reducing the preparation time without decreasing the quality of the results. Microwave irradiation proved to be quite efficient and reliable for this purpose, as it allowed to identify Ag-NORs equivalent to those obtained by the conventional procedure and also to reduce the concentration of the employed reagents, as well as the precipitation of debris on the preparation.

【 授权许可】

CC BY   
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