Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum

Genetics and Molecular Biology

Eliane P. Cervelatti¹  Monica S. Ferreira-nozawa¹  Roseli Aquino-ferreira¹  Ana Lúcia Fachin¹  Nilce M. Martinez-rossi¹ 

[1] ,Universidade de São Paulo Faculdade de Medicina de Ribeirão Preto Departamento de GenéticaRibeirão Preto SP ,Brazil

关键词: Trichophyton rubrum;    fungal chromosomes;    electrophoretic karyotype;    dermatophyte;    contour-clamped homogeneous electric field;   

DOI  :  10.1590/S1415-47572004000100016

来源: SciELO

【 摘 要 】

The electrophoretic karyotype of the dermatophyte Trichophyton rubrum was established using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Five chromosomal bands of approximately 3.0 to 5.8 megabase pairs (Mbp) each were observed and together indicated that 22.05 Mbp of the total genome are organized as chromosomal macromolecules. In addition to establishing the number and size of T. rubrum chromosomes, these results open perspectives for the construction of chromosome-specific libraries and for the physical mapping of genes of interest, thus permitting future gene linkage studies in this pathogen. A detailed understanding of the karyotype and genomic organization of T. rubrum should contribute to further genetic, taxonomic and epidemiological studies of this dermatophyte.

【 授权许可】

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