Hepatic mRNA expression and plasma levels of insulin-like growth factor-I (IGF-I) in broiler chickens selected for different growth rates

Genetics and Molecular Biology

Poliana Fernanda Giachetto²  Eduardo Correa Riedel²  Jane Eyre Gabriel²  Maria Inês Tiraboschi Ferro²  Sônia Marli Zingaretti Di Mauro²  Marcos Macari¹  Jesus Aparecido Ferro² 

[1] ,Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de TecnologiaJaboticabal SP ,Brazil

关键词: IGF-I mRNA;    broiler chicken;    growth performance;    plasma IGF-I;   

DOI  :  10.1590/S1415-47572004000100007

来源: SciELO

【 摘 要 】

The hepatic expression and plasma concentrations of IGF-I were investigated in three broiler chicken strains selected for different growth rates (HP-Hubbard-Pettersen, a fast growing strain; NN-Naked-neck, a strain with an intermediate growth rate and a heterozygous genotype, and C-Caipira, a slow growing crossbred strain). The chickens were studied at 1, 21 and 42 days of age and had free access to food throughout the study. Hepatic IGF-I mRNA expression was assessed by dot blot analysis using a randomly labeled chicken IGF-I cDNA as the probe and plasma IGF-I concentrations were assayed by radioimmunoassay. The hepatic levels of IGF-I mRNA increased from 1 to 21 days of age in all strains, with NN chickens showing a higher (p < 0.05) IGF-I expression than the other strains. Plasma IGF-I concentrations increased (p < 0.05) with broiler chicken age, but there were no significant differences among the strains. These results indicate that despite differences in the growth rates among the strains, the changes in the expression of IGF-I mRNA in liver and in the plasma levels of IGF-I were independent of broiler chicken strain, but varied with chicken age.

【 授权许可】

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