Comparative molecular analysis of Herbaspirillum strains by RAPD, RFLP, and 16S rDNA sequencing

Genetics and Molecular Biology

Juliana R.l. Soares-ramos¹  Humberto J.o. Ramos¹  Leonardo M. Cruz¹  Leda S. Chubatsu¹  Fábio O. Pedrosa¹  Liu U. Rigo¹  Emanuel M. Souza¹ 

[1] ,Universidade Federal do Paraná Departamento de Bioquímica e Biologia Molecular Curitiba PR ,Brazil

关键词: Herbaspirillum;    RAPD;    RFLP;    Phylogeny;    16S rDNA;   

DOI  :  10.1590/S1415-47572003000400019

来源: SciELO

【 摘 要 】

Herbaspirillum spp. are endophytic diazotrophic bacteria associated with important agricultural crops. In this work, we analyzed six strains of H. seropedicae (Z78, M2, ZA69, ZA95, Z152, and Z67) and one strain of H. rubrisubalbicans (M4) by restriction fragment length polymorphism (RFLP) using HindIII or DraI restriction endonucleases, random amplified polymorphic DNA (RAPD), and partial sequencing of 16S rDNA. The results of these analyses ascribed the strains studied to three distinct groups: group I, consisting of M2 and M4; group II, of ZA69; and group III, of ZA95, Z78, Z67, and Z152. RAPD fingerprinting showed a higher variability than the other methods, and each strain had a unique electrophoretic pattern with five of the six primers used. Interestingly, H. seropedicae M2 was found by all analyses to be genetically very close to H. rubrisubalbicans M4. Our results show that RAPD can distinguish between all Herbaspirillum strains tested.

【 授权许可】

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