DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars

Genetics and Molecular Biology

S.s. Sandhu²  Carlos Colombo¹  Cândido R. Bastos¹  Walter J. Siqueira¹ 

[1] ,R.D. University Department of Biological Sciences Jabalpur,India

关键词: rice;    SCAR;    arbitrary primers;    Pyricularia oryzae;   

DOI  :  10.1590/S1415-47572003000400011

来源: SciELO

【 摘 要 】

Rice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines which differed from susceptible lines. By comparing different susceptible lines, nine DNA amplifications of seven primers (OPA5(1000), OPA5(1200,) OPG17(700), OPG18(850), OPG19(500), OPG19(600), OPF9(600), OPF17(1200) and OPF19(600)) were identified as dominant markers for the blast resistant gene in resistant cultivar lines. These loci facilitate the indirect scoring of blast resistant and blast susceptible genotypes. The codomine RAPDs markers will facilitate marker-assisted selection of the blast resistant gene in two blast resistant genotypes of rice (Labelle and Line 11) and will be useful in rice breeding programs.

【 授权许可】

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