Genetics and Molecular Biology | |
Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers | |
Maria Imaculada Zucchi2  Rosana Pereira Vianello Brondani1  José Baldin Pinheiro1  Lázaro José Chaves1  Alexandre Siqueira Guedes Coelho1  Roland Vencovsky2  | |
[1] ,Universidade de São Paulo ESALQ Departamento de GenéticaPiracicaba SP ,Brazil | |
关键词: SSR; Myrtaceae; Cerrado; tropical tree; genetic diversity and transferability; | |
DOI : 10.1590/S1415-47572003000400008 | |
来源: SciELO | |
【 摘 要 】
The ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including expected average heterozygosity He = 0,442, among population diversity, R ST = 0,268 and gene flow Nm = 0,680. Results indicated a potential of SSR locus transferability developed for Eucalyptus to other species of different genera, such as in the case of the ''cagaita tree''. The high genetic diversity among populations detected with SSR markers indicated that these markers are highly sensitive to detect population structure. Estimated Nm values and the existence of private alleles indicated reduced gene flow and consequently possible damage to the metapopulation structure.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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