Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers

Genetics and Molecular Biology

Maria Imaculada Zucchi²  Rosana Pereira Vianello Brondani¹  José Baldin Pinheiro¹  Lázaro José Chaves¹  Alexandre Siqueira Guedes Coelho¹  Roland Vencovsky² 

[1] ,Universidade de São Paulo ESALQ Departamento de GenéticaPiracicaba SP ,Brazil

关键词: SSR;    Myrtaceae;    Cerrado;    tropical tree;    genetic diversity and transferability;   

DOI  :  10.1590/S1415-47572003000400008

来源: SciELO

【 摘 要 】

The ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including expected average heterozygosity He = 0,442, among population diversity, R ST = 0,268 and gene flow Nm = 0,680. Results indicated a potential of SSR locus transferability developed for Eucalyptus to other species of different genera, such as in the case of the ''cagaita tree''. The high genetic diversity among populations detected with SSR markers indicated that these markers are highly sensitive to detect population structure. Estimated Nm values and the existence of private alleles indicated reduced gene flow and consequently possible damage to the metapopulation structure.

【 授权许可】

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