Cytochrome P450c17alpha (CYP17) gene polymorphism is not associated with leiomyoma susceptibility

Genetics and Molecular Biology

Hsieh Yao-yuan²  Tsai Fuu-jen¹  Chang Chi-chen²  Tsai Chang-hai¹  Lin Cheng-chieh¹  Yeh Lian-shun² 

[1] ,China Medical College Hospital Department of Obstetrics and Gynecology

关键词: cytochrome P450c17;    CYP17;    leiomyoma;    single nucleotide polymorphism;   

DOI  :  10.1590/S1415-47572002000400002

来源: SciELO

【 摘 要 】

Estrogen plays a role in the pathogenesis of leiomyoma. The CYP17 gene codes for the cytochrome P450c17alpha enzyme, which is involved in the biosynthesis of estrogen. Our aim was to investigate if CYP17 polymorphism could be a useful marker to predict the susceptibility to leiomyoma. Our sample of female subjects was divided into two groups: (1) with leiomyoma (n = 159); (2) without leiomyoma (n = 128). A 169-bp fragment encompassing the A1/A2 polymorphic site of the CYP17 gene was amplified by polymerase chain reaction (PCR), restricted by enzyme MspA1I and electrophored on agarose gel. Genotypes and allelic frequencies for this polymorphism in both groups were compared. There was no significant difference between the two groups regarding the distribution of the CYP17 gene polymorphism frequencies. The A1 homozygote/heterozygote/A2 homozygote proportions for CYP17 in both groups were: (1) 17.0/46.5/36.5%, and (2) 17.2/45.3/37.5%. The proportions for alleles A1 and A2 were also comparable in the two groups. A1 and A2 allele frequencies were: 7% (40.3/59) in group 1, and 2% (39.8/60) in group 2. No significant association was observed between the risk of leiomyoma and polymorphisms of the CYP 17 gene. So, CYP17 gene polymorphism does not appear to be a useful marker for the prediction of leiomyoma susceptibility.

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