Late-replicating X-chromosome: replication patterns in mammalian females

Genetics and Molecular Biology

Karen Tunin¹  Priscila Guimarães Otto¹ 

[1] ,Universidade de São Paulo Instituto de Biociências Departamento de BiologiaSão Paulo,Brazil

关键词: X-chromosome;    replication patterns;    mammalian females;   

DOI  :  10.1590/S1415-47572002000300009

来源: SciELO

【 摘 要 】

The GTG-banding and 5-BrdU incorporation patterns of the late-replicating X-chromosome were studied in female dogs and cattle, and compared to human female patterns. The replication patterns of the short arm of the X-chromosomes did not show any difference between human, dog and cattle females. As to the long arm, some bands showed differences among the three studied species regarding the replication kinetics pattern. These differences were observed in a restricted region of the X-chromosome, delimited by Xq11 -> q25 in humans, by Xq1 -> q8 in dogs, and by Xq12 -> q32 in cattle. In an attempt to find out if these differences in the replication kinetics could be a reflection of differences in the localization of genes in that region of the X-chromosome, we used the probe for the human androgen receptor gene (AR) localized at Xq12, which is in the region where we observed differences among the three studied species. We did not, however, observe hybridization signals. Our study goes on, using other human probes for genes located in the region Xq11 -> Xq25.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License