Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysis

Genetics and Molecular Biology

Ana Maria Pujol Vieira Dos Santos²  Aida T. Santos Matsumura²  Sueli Teresinha Van Der Sand¹ 

[1] ,Universidade Federal do Rio Grande do Sul Departamento de Fitossanidade Porto Alegre RS ,Brazil

关键词: Drechslera tritici-repentis;    RAPD;    DNA;    polymorphism;    genetic diversity;   

DOI  :  10.1590/S1415-47572002000200020

来源: SciELO

【 摘 要 】

The phytopathogenic fungus Drechslera tritici-repentis causes tan spot, an important disease of wheat in the southern Brazilian state of Rio Grande do Sul. Twelve D. tritici-repentis isolates were obtained from wheat seeds from different locations in the state. Their colony morphology on potato dextrose agar and polymorphisms in genomic DNA by the random amplified polymorphic DNA (RAPD) method were investigated. For the RAPD method, 23 primers were tested of which nine were selected for use in the study of D. tritici-repentis polymorphisms. The degree of similarity between isolates was calculated using a simple matching coefficient and dendrograms constructed by the unweighted pair-group method with arithmetical averages (UPGMA). The morphological and RAPD analyses showed intraspecific polymorphisms within the isolates, but it was not possible to establish a relationship between these polymorphisms and the geographical regions from where the host seeds were collected.

【 授权许可】

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