Genetic instability of sugarcane plants derived from meristem cultures

Genetics and Molecular Biology

Maria Imaculada Zucchi²  Hideto Arizono¹  Vicente Alberto Morais¹  Maria Helena Pelegrinelli Fungaro¹  Maria Lúcia Carneiro Vieira² 

[1] ,Escola Superior de Agricultura "Luiz de Queiroz" Departamento de Genética

关键词: sugarcane;    somaclonal variation;    meristem cultures;    phenotypic instability;    RAPD;   

DOI  :  10.1590/S1415-47572002000100017

来源: SciELO

【 摘 要 】

The RADP (Random amplified polymorphic DNA) technique was used to detect tissue-culture-induced variations in sugarcane. Plants of the Brazilian variety RB83-5486 propagated via rhizomes and via meristem cultures were studied. The polymorphism rate for 98 RAPD loci was 6.93% when the plants derived from meristems. Besides, in order to evaluate the influence of the number of subcultures on the generation of somaclonal variation, field-grown RB83-5486 plants derived from 10 meristems were studied after five subcultivations. Although different rates of polymorphism were observed, there was no direct association with the stage of subcultivation. The analysis of plants of two sugarcane varieties cultivated in vitro from meristems showed that variety RB83-5486 was more unstable than variety SP80-185.

【 授权许可】

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