Variability among inbred lines and RFLP mapping of sunflower isozymes

Genetics and Molecular Biology

Alicia D. Carrera²  G. Pizarro²  M. Poverene²  S. Feingold¹  A.j. León¹  S.t. Berry¹ 

[1] ,Universidad Nacional del Sur Departamento de Agronomía y CERZOS Bahía Blanca,Argentina

关键词: isozyme;    polymorphisms;    RFLP markers;    sunflower;    linkage map;    Helianthus annuus;   

DOI  :  10.1590/S1415-47572002000100013

来源: SciELO

【 摘 要 】

Eight isozyme systems were used in this study: acid phosphatase (ACP), alcohol dehydrogenase (ADH), esterase (EST), glutamate dehydrogenase (GDH), malate dehydrogenase (MDH), phosphoglucoisomerase (PGI), 6-phosphogluconate dehydrogenase (PGD), and phosphoglucomutase (PGM). The polymorphism of these enzyme systems was studied in 25 elite inbred lines. A total of 19 loci were identified, but only eight of them were polymorphic in the germplasm tested. The polymorphic index for the eight informative markers ranged from 0.08 to 0.57, with a mean value of 0.36. Five isozyme loci were mapped in F2:3 populations with existing RFLP data. Est-1, Gdh-2 and Pgi-2 were mapped to linkage groups 3, 14 and 9, respectively. As in previous reports, an ACP locus and a PGD locus were found to be linked, both located in linkage group 2 of the public sunflower map.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License