Comparison of immunoperoxidase and immunofluorescence assays for pp65 cytomegalovirus antigen in immunocompromised patients

Brazilian Journal of Infectious Diseases

Maria Do Carmo Debur¹  Luine R. Rosele Vidal¹  Meri Bordignon Nogueira¹  Luciane Aparecida Pereira¹  Gislene R.a. Takahashi¹  Indianara Rotta¹  Sérgio Monteiro De Almeida¹  Sonia Mara Raboni¹ 

[1] ,Universidade Federal do Paraná Hospital de Clínicas Virology LaboratoryCuritiba PR ,Brazil

关键词: Immunoperoxidase;    immunofluorescence;    cytomegalovirus;    immunocompromised patients;    bone marrow transplantation;    solid organ transplant;   

DOI  :  10.1590/S1413-86702009000200014

来源: SciELO

【 摘 要 】

We compared the pp65 antigen detection by an in house method (immunoperoxidase assay) and by a commercial kit (immunofluorescence assay) available for cytomegalovirus infection diagnosis in immunocompromised patients. Sixty-four blood samples were analyzed in duplicate for both techniques. Eight-six percent of the samples had concordant qualitative results. The discordant results occurred more frequently in samples with low quantity of positive cells. There were no significant differences with qualitative and quantitative results of the methods.

【 授权许可】

CC BY-NC-ND   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License