Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci

Brazilian Journal of Infectious Diseases

Carina Secchi²  Ana Lúcia Souza Antunes²  Leandro Reus Rodrigues Perez²  Vlademir Vicente Cantarelli¹  Pedro Alves D'azevedo² 

[1] ,Federal University of Health Sciences of Porto Alegre

关键词: Coagulase-negative Staphylococcus;    cefoxitin;    oxacillin;    mecA gene;    susceptibility diagnostic;   

DOI  :  10.1590/S1413-86702008000400012

来源: SciELO

【 摘 要 】

The NCCLS (2004) presented a new methodology to detect, by disk-diffusion agar, oxacillin-resistance using a cefoxitin disk. We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 µg) with oxacillin disks (1 µg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-negative bacteria other than Staphylococcus epidermidis (SCoNne). A total of 238 SCoNne was evaluated; oxacillin-resistance (the mecA gene) was detected in 71% of the isolates. All methods gave 100% sensitivity, based on presence of the mecA gene. The specificity of the cefoxitin disk was 100%, while the oxacillin disk gave a specificity of 91% and agar dilution oxacillin gave a specificity of 88%. We conclude that the cefoxitin disk is an efficient test, and it is an easy method for use in clinical laboratories to detect oxacillin-resistance in staphylococci.

【 授权许可】

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