期刊论文详细信息
Brazilian Journal of Infectious Diseases
Preliminary evaluation of adherence on abiotic and cellular surfaces of Acinetobacter baumannii strains isolated from catheter tips
Gislaine Franco De Moura Costa2  Maria Cristina Bronharo Tognim1  Celso Luíz Cardoso1  Floristher Elaine Carrara-marrone1  Lourdes Botelho Garcia1 
[1] ,State University of West of Paraná Center of Medical and Pharmaceutical Sciences Cascavel PR ,Brazil
关键词: Acinetobacter baumannii;    hydrophobicity;    adherence;   
DOI  :  10.1590/S1413-86702006000500009
来源: SciELO
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【 摘 要 】

The cell surface hydrophobicity and adhesion to abiotic and cellular surfaces was tested in five clinical strains of Acinetobacter baumannii isolated from catheter tips. Biochemical and molecular characteristics of these strains were also studied. Hydrophobicity was characterized by a test for affinity to xylene. Adhesion to abiotic surfaces (polystyrene, formica, latex and glass) was evaluated in Petri plates using the stamp technique. Buccal epithelial cells were used for tests of adhesion to cellular surfaces. Adhesion to the catheter was evaluated by repeatedly rinsing the catheters and rolling them over nutrient agar. Molecular typing of the strains was done by the ERIC-PCR technique. The degree of hydrophobicity of the strains varied from hydrophobic to hydrophilic. All the strains adhered to the cell surfaces and to the catheters, and three of them strongly adhered to latex, polystyrene and formica. Catheter adhesion was reduced by meropenem. We found a direct relationship between the degree of bacterial hydrophobicity and adhesion to the abiotic surfaces, but not with adhesion to cellular surfaces, which suggests that different mechanisms are involved in adherence.

【 授权许可】

CC BY-NC-ND   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License

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