RICE PARBOILING WASTEWATER IN THE MAXIMIZATION OF CAROTENOIDS BIOPRODUCTION BY Phaffia rhodozyma

Ciência e Agrotecnologia

Danielle Alves Da Silva Rios¹  Thais De Matos De Borba¹  Susana Juliano Kalil¹  Janaína Fernandes De Medeiros Burkert¹ 

关键词: Biodye;    submerged cultive;    response surface methodology;    yeast;    Biocorantes;    cultivo submerso;    superfície de resposta;    levedura;   

DOI  :  10.1590/S1413-70542015000400011

来源: SciELO

【 摘 要 】

The interest in carotenoid production from natural sources has increased based in their possible use as dyes and their powerful biological antioxidant capacity. This study evaluated the use of rice parboiling wastewater (RPW) as an alternative substrate for the bioproduction of carotenoids using the yeast Phaffia rhodozyma and found it to be promising as the only source of nutrients, reaching a concentration of 0.6 μg mL-1 (259.1 μg g-1) in 48 h. To increase the potential use of this industrial effluent, a study of supplementation was carried out to enhance the production of carotenoids using the methodology of experimental design (a 2IV 6-2 fractional factorial design sequenced by a 24 central composite design). The conditions for maximizing the production of carotenoids were (g L-1) malt extract (16.25), peptone (8.75), sucrose (15) and rice parboiling wastewater (87.5), with a pH of 5 at 25 °C and 150 rpm for 144 h, which produced a concentration 5.3 μg mL-1 (628.8 μg g-1).

【 授权许可】

CC BY   
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