Ciência e Agrotecnologia | |
Production of lipase extrated from aqueous waste: enzymatic activity kinetics | |
Tatianne Ferreira De Oliveira2  Maria Raquel Hidálgo1  Manoel Soares Soares Júnior2  | |
[1] ,Universidade Federal de Goiás Escola de Agronomia e Engenharia de Alimentos Goiânia GO ,Brasil | |
关键词: Microbial extract; effluent treatment; sustainability; Extrato microbiano; tratamento de efluentes; sustentabilidade; | |
DOI : 10.1590/S1413-70542014000600005 | |
来源: SciELO | |
【 摘 要 】
Lipases are an important group of enzymes with various applications in the food, chemical and pharmaceutical industry, besides having great interest for the treatment of effluents with high lipid content. The objective of this study was to isolate, characterize and select lipolytic bacteria that produce lipase from aqueous waste effluents and to study the enzymatic activity kinetics of the extract obtained via submerged fermentation. The results obtained are promising, being possible to isolate and characterize 23 lipase-producing microorganisms, mostly gram-positive bacteria, but after the fermentation step in a liquid medium, gram negative bacteria showed the highest enzymatic activity (56.72 U.L-1 for STP 2A` bacterium and 81.99 U.L-1 for R2B). In the enzymatic activity kinetic study with the selected bacterium (R2B), among the six variables (temperature, pH, minimal mineral medium, soybean oil, glucose and sodium nitrate), temperature was the one that most positively influenced the enzymatic activity, and the best results were obtained at 40°C. It was concluded that the enzyme extract obtained from environmental waste may be used to treat the effluent and contribute to reduce environmental impacts.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202005130138347ZK.pdf | 928KB | download |