Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp

Ciência e Agrotecnologia

Ana Luiza De Oliveira Timbó²  Roselaine Cristina Pereira¹  Vanderley Borges Dos Santos¹  Fausto Souza Sobrinho¹  Lisete Chamma Davide¹ 

[1],Universidade Federal de Lavras Departamento de Agricultura Lavras MG ,Brasil

关键词: Brachiaria ruziziensis;    Brachiaria brizantha;    colchicine;    nuclear DNA content;    Brachiaria ruziziensis;    Brachiaria brizantha;    colchicina;    conteúdo de DNA nuclear;   

DOI  :  10.1590/S1413-70542012000600001

来源: SciELO

【 摘 要 】

Flow cytometry allows to estimate the DNA content of a large number of plants quickly. However, inadequate protocols can compromise the reliability of these estimates leading to variations in the values of DNA content the same species. The objective of this study was to propose an efficient protocol to estimate the DNA content of Brachiaria spp. genotypes with different ploidy levels using flow cytometry. We evaluated four genotypes (B. ruziziensis diploid and artificially tetraploidized; a tetraploid B. brizantha and a natural triploid hybrid), three buffer solutions (MgSO4, Galbraith and Tris-HCl) and three species as internal reference standards (Raphanus sativus, Solanum lycopersicum e Pisum sativum). The variables measured were: histogram score (1-5), coefficient of variation and estimation of DNA content. The best combination for the analysis of Brachiaria spp. DNA content was the use of MgSO4 buffer with R. sativus as a internal reference standard. Genome sizes expressed in picograms of DNA are presented for all genotypes and the importance of the histogram score on the results reliability of DNA content analyses were discussed.

【 授权许可】

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