Effect of different carbon sources on the in vitro multiplication of Annona sp.

Ciência e Agrotecnologia

José Raniere Ferreira De Santana²  Renato Paiva¹  Ana Valéria De Souza¹  Lenaldo Muniz De Oliveira¹ 

[1] ,Universidade Estadual de Feira de Santana Departamento de Biologia Laboratório de Cultura de TecidosFeira de Santana BA

关键词: Annonaceae;    micropropagation;    carbohydrates;    Annonaceae;    micropropagação;    carboidratos;   

DOI  :  10.1590/S1413-70542011005000002

来源: SciELO

【 摘 要 】

The Annonaceae family comprises approximately 2.300 species, some with significant commercial value. Although commercial plantations have suffered due to problems related to seedling production. As micropropagation is a viable technique for seedling production, the present work evaluated the effects of different carbon sources on in vitro bud induction in five Annonaceae species. Nodal segments obtained from plants of the Annona glabra, A. cauliflora, A. coriacea, A. bahiensis and Rollinia silvatica species were inoculated into solid WPM culture medium with 8.87 μM BAP, 0.86 mM of benomyl, and 87.64 mM of the following carbon sources: glucose, sucrose, fructose, galactose, sorbitol and maltose. We evaluated the buds number, the length and weight of the largest bud, the number of expanded leaves per bud, the length of the largest leaf and the dry matter of the buds. No significant difference was observed among the different carbon sources used in terms of the number of produced buds; however, the length of the largest bud, the number of expanded leaves, the length of the largest leaf, and dry weight of the buds presented significant difference according to the studied speciesas well as the carbon sources used, with the lowest value being obtained with sorbitol. The results obtained here indicated that, except for sorbitol, any of the carbohydrates tested could be used in the in vitro multiplication protocols for A. bahiensis, A. cauliflora, A. coriacea, A. glabra and R. silvatica.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License