Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.

Ciência e Agrotecnologia

Maria Gabriela Bello Koblitz²  Gláucia Maria Pastore¹ 

[1] ,Universidade Estadual de Feira de Santana Faculdade de Engenharia de Alimentos DTEC

关键词: Lipase;    Rhizopus sp.;    purification;    biochemical characterization;    Lipase;    Rhizopus sp.;    purificação;    caracterização bioquímica;   

DOI  :  10.1590/S1413-70542006000300016

来源: SciELO

【 摘 要 】

The present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg+2 and by n-bromosuccinimide and activated by Na+.

【 授权许可】

CC BY   
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