Effects of low-intensity pulsed ultrasound on injured skeletal muscle

Brazilian Journal of Physical Therapy

Camila S. Montalti¹  Natália V. C. K. L. Souza¹  Natália C. Rodrigues¹  Kelly R. Fernandes¹  Renata L. Toma¹  Ana C. M. Renno¹ 

关键词: muscle tissue;    muscle injury;    cryolesion;    muscle regeneration;    low-intensity pulsed ultrasound;   

DOI  :  10.1590/S1413-35552012005000101

来源: SciELO

【 摘 要 】

BACKGROUND:Low-intensity pulsed ultrasound (LIPUS) has been shown to stimulate tissue metabolism and accelerate muscle healing. However, the optimal parameters in the use of LIPUS are still not clear.OBJECTIVE:The aim of this study was to analyze the effects of LIPUS on muscle healing in rats subjected to a cryolesion.METHOD:Twenty rats were divided into the following groups: an injured control group (CG) and an injured treated group (TG). Both groups were divided into 2 sub-groups (n=5 each) that were sacrificed 7 and 13 days post-surgery. Treatments were started 24 hours after the surgical procedure and consisted of 3 or 6 sessions. After euthanasia, the muscles were submitted to standard histological procedures.RESULTS:Qualitative analyses were based on morphological assessments of the muscle. The histopathological analysis on day 7 revealed that the muscles in the CG and the TG presented an intense inflammatory infiltrate, a large necrotic area and a disorganized tissue structure. After 13 days, both the CG and the TG had granulation tissue and newly formed fibers. The TG presented a more organized tissue structure. The quantitative analysis of collagen indicated similar findings among the groups, although the qualitative analysis revealed a better organization of collagen fibers in the TG at 13 days. The immunohistochemical analysis indicated that, at both time points, the expression of cyclooxygenase-2 was upregulated in the TG compared to the CG.CONCLUSIONS: LIPUS used as a treatment for muscle injury induced a more organized tissue structure at the site of the injury and stimulated the expression of COX-2 and the formation of new muscle fibers.

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