The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients*

Anais Brasileiros de Dermatologia

Katia Cristina Dantas¹  Roseli S. Freitas¹  Adriana Pardini Vicentini Moreira¹  Marcos Vinicius Da Silva¹  Gil Benard¹  Cidia Vasconcellos¹  Paulo Ricardo Criado¹ 

关键词: AIDS-related opportunistic infections;    Histoplasma;    HIV seropositivity;    Molecular diagnostic techniques;    Polymerase chain reaction;    Histoplasma;    Infecções oportunistas relacionadas com a AIDS;    Reação em cadeia da polimerase;    Soropositividade para HIV;    Técnicas de diagnóstico molecular;   

DOI  :  10.1590/S0365-05962013000100025

来源: SciELO

【 摘 要 】

The aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum: 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8). The PCR sequences HC18, HC100 and HC5.8 resulted in a specificity of 100%. The molecular assays may increase the specificity, sensitivity and speed in the diagnosis of Histoplasmosis.

【 授权许可】

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