Techniques of digital image analysis for histological quantification of melanin

Anais Brasileiros de Dermatologia

Hélio Amante Miot²  Gabrielli Brianezi¹  Andréia De Almeida Tamega¹  Luciane Donida Bartoli Miot² 

[1] ,Universidade Estadual Paulista Julio de Mesquita Filho School of Medicine of Botucatu Department of Dermatology and RadiotherapySao Paulo SP ,Brazil

关键词: Cluster analysis;    Image cytometry;    Melanin;    Melanosis;    Pigmentation disorders;    Análise por conglomerados;    Citometria por imagem;    Melaninas;    Melanose;    Transtornos da pigmentação;   

DOI  :  10.1590/S0365-05962012000400014

来源: SciELO

【 摘 要 】

Morphometric analysis of tissue melanin may quantitatively contribute to research on pigmentation disorders. The authors present three methods for image analysis, which allow for identification of melanin-equivalent pixels in the epidermis using Fontana-Masson stain and, therefore, for the calculation of its percentage in the different epidermal layers. Moreover, they discuss the main elements related to the analysis and the need for rigorous standardization of the process.

【 授权许可】

CC BY-NC   
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