Immunoelectron microscopy study of superficial skin nerves in drug-induced acute urticaria

Anais Brasileiros de Dermatologia

Paulo Ricardo Criado²  Roberta Fachini Jardim Criado¹  Cleusa F.h. Takakura¹  Carla Pagliari¹  Mirian Nacagami Sotto¹  Cidia Vasconcellos¹ 

[1],São Paulo University School of Medicine Dermatology DepartmentSão Paulo SP ,Brazil

关键词: factor XIIIa;    mast cells;    neurons;    tryptases;    urticaria;    fator XIIIa;    mastócitos;    neurônios;    triptases;    urticária;   

DOI  :  10.1590/S0365-05962012000300004

来源: SciELO

【 摘 要 】

BACKGROUND: Few studies have evaluated the ultrastructure of the superficial skin nerves in urticaria. OBJECTIVE: The objective of this study was to describe findings in superficial skin nerves in cases of drug-induced acute urticaria. METHODS: Seven patients with drug-induced acute urticaria were included in the study. Skin biopsies were obtained from the urticarial lesion and from the apparently normal skin. The 14 fragments collected were processed for immunogold electron microscopy using single stains for antitryptase and anti-FXIIIa antibodies, as well as double immunogold labeling for both. RESULTS: Some sections showed mast cells in the process of degranulation. Following double immunogold staining, 10 nm (FXIIIa) and 15 nm (Tryptase) gold particles were found together throughout the granules in mast cells, indicating that tryptase and FXIIIa are located inside each one of the granules of these cells. Interestingly, we found strong evidence of the presence of tryptase and factor XIIIa in the superficial skin nerves of these patients, both in cases of urticarial lesions (wheals) and in the apparently normal skin. CONCLUSIONS: Tryptase and FXIIIa are present in the superficial nerves of the skin in drug-induced acute urticaria. This is the first report of tryptase and FXIIIa expression in the superficial skin nerves of patients with urticaria. Tryptase may be participating in neural activation in these patients, while FXIIIa may be present in the nerves to guarantee the functional integrity of structures.

【 授权许可】

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