PERFORMANCE OF LOOFA-IMMOBILIZED Rhizopus oryzae IN THE ENZYMATIC PRODUCTION OF BIODIESEL WITH USE OF OLEIC ACID IN n-HEXANE MEDIUM

Brazilian Journal of Chemical Engineering

S. Sattari¹  F. Vahabzadeh¹  H. K. Aghtaei¹ 

关键词: Rhizopus oryzae;    Cell-bound lipases;    Loofa sponge;    Methyl oleate;    n-hexane.;   

DOI  :  10.1590/0104-6632.20150322s00003525

来源: SciELO

【 摘 要 】

AbstractThe performance of loofa-immobilized Rhizopus oryzae (as a whole-cell biocatalyst) in the synthesis of methyl oleate was evaluated using oleic acid as a model substrate. The activities of the cell-bound lipase in terms of the esterification and hydrolysis reactions were found to be higher for the immobilized cells as compared with those of the free cells. The time to reach equilibrium for methyl oleate synthesis was 12 h in the presence of n-hexane (hexane:oleic acid ratio 9:1(v/v)), and the yield was 80%. In the absence of solvent, equilibrium was reached after 48 h and the yield was only 30%. The moisture repellency and the hydrophilic properties of loofa sponge make this natural fiber a good candidate for cell-enzyme immobilization, especially for lipases as the interfacial enzyme.

【 授权许可】

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