Influence of bloom number and plastifiers on gelatin matrices produced for enzyme immobilization

Brazilian Journal of Chemical Engineering

A. P. Kempka²  S. M. A. G. Ulson De Souza¹  A. A. Ulson De Souza¹  R. C. Prestes¹  D. Ogliari² 

[1],State University of Santa Catarina Food Engineering Department Pinhalzinho SC ,Brazil

关键词: Gelatin;    Glycerol;    Mannitol;    Immobilization;    Enzyme;   

DOI  :  10.1590/S0104-66322014000100010

来源: SciELO

【 摘 要 】

In this study the immobilization of lipase in matrices produced with gelatin with different Bloom values and with the addition of plastifiers was investigated to evaluate the influence of the Bloom value, as well as the capacity of the plastifiers to maintain the enzyme immobilized and the immobilization yield. The results indicated the need for crosslinking of the matrices with glutaraldehyde due to the high solubility in water, explained by the amino acid profile, which confirms the solubility of gelatin. Mannitol showed greater efficiency in the lipase immobilization, since it led to more porous structures and more uniform pores. These structures were also influenced by the gelatin concentration; greater concentrations associated with intermediate concentrations of plastifier led to matrices with a greater immobilization yield (87.92%). The X-ray diffraction analysis revealed that the structure of the immobilization matrices was partially crystalline.

【 授权许可】

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