Kinetic study of the enzymatic hydrolysis of sugarcane bagasse

Brazilian Journal of Chemical Engineering

M. L. Carvalho²  R. Sousa Jr.²  U. F. Rodríguez-zúñiga²  C. A. G. Suarez²  D. S. Rodrigues¹  R. C. Giordano²  R. L. C. Giordano² 

[1] ,Federal University of São Carlos Department of Chemical Engineering São Carlos SP ,Brazil

关键词: Cellulose enzymatic hydrolysis;    Sugarcane bagasse;    Kinetic study;   

DOI  :  10.1590/S0104-66322013000300002

来源: SciELO

【 摘 要 】

This work presents a kinetic study of the enzymatic hydrolysis of three cellulosic substrates: filter paper (FP), used as a low recalcitrance substrate model; steam exploded sugarcane bagasse (SB); and weak acid pretreated SB (1:20 dry bagasse:H2SO4 solution 1% w/w), the last two delignified with 4% NaOH (w/w). The influence of substrate concentration was assessed in hydrolysis experiments in a shaker, using Accellerase® 1500, at pH 4.8, in 50 mM sodium citrate buffer. Cellulose loads (weight substrate/weight total) were changed between 0.5%-13% (for FP) and 0.99%-9.09% (for SB). For FP and low loads of steam exploded SB, it was possible to fit pseudo-homogeneous Michaelis-Menten models (with inhibition). For FP and higher loads of steam exploded SB, modified Michaelis-Menten models were fitted. Besides, it was observed that, after retuning of the model parameters, it is possible to apply a model fitted for one situation to a different case. Chrastil models were also fitted and they were the only feasible approach for the highly recalcitrant acid-treated SB.

【 授权许可】

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