Optimization of the precipitation of clavulanic acid from fermented broth using t-octylamine as intermediate

Brazilian Journal of Chemical Engineering

D. B. Hirata²  J. H. H. L. Oliveira²  K. V. Leão¹  M. I. Rodrigues¹  A. G. Ferreira¹  M. Giulietti¹  M. Barboza¹  C. O. Hokka¹ 

[1] ,Universidade Federal de Alfenas Institute of Chemistry Alfenas MG ,Brasil

关键词: Clavulanic acid;    Precipitation reaction;    Potassium 2-ethylhexanoate;    T-octylamine;   

DOI  :  10.1590/S0104-66322013000200002

来源: SciELO

【 摘 要 】

This work describes the use of clavulanic acid (CA) precipitation as the final step in the process of purification of CA from fermentation broth as an alternative to conventional methods employed traditionally. The purpose of this study was to use a stable intermediate (t-octylamine) between the conversion of CA to its salt form (potassium clavulanate), thereby enabling the resulting intermediate (amine salt of clavulanic acid) to improve the purification process and maintain the stability of the resulting potassium clavulanate. To this end, response surface methodology was employed to optimize the precipitation step. For the first reaction, five temperatures (6.6 to 23.4 ºC), concentrations of clavulanic acid in organic solvent (6.6 to 23.4 mg/mL) and t-octylamine inflow rates (0.33 to 1.17 drop/min) were selected based on a central composite rotatable design (CCRD). For the second reaction, five temperatures (11.6 to 28.4 ºC), concentrations of clavulanic acid amine salt in organic solvent (8.2 to 41.8 mg/mL) and concentrations of potassium 2-ethylhexanoate (0.2 to 1.2 molar) were also selected using CCRD. From these results, precipitation conditions were selected and applied to the purification of CA from the fermentation broth, obtaining a yield of 72.37%.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License