Ethanol production from sorghum grains [Sorghum bicolor (L.) Moench]: evaluation of the enzymatic hydrolysis and the hydrolysate fermentability

Brazilian Journal of Chemical Engineering

C. A. Barcelos¹  R. N. Maeda¹  G. J. V. Betancur¹  N. Pereira Jr.¹ 

[1] ,Federal University of Rio de Janeiro Center of Technology Department of Biochemical EngineeringRio de Janeiro RJ ,Brazil

关键词: Sorghum Grains;    Enzymatic Hydrolysis;    Ethanol;    Fermentation;    Saccharomyces cerevisiae;   

DOI  :  10.1590/S0104-66322011000400005

来源: SciELO

【 摘 要 】

The production of ethanol from sorghum grains was investigated in the present work. Initially, starch enzymatic hydrolysis was investigated using commercial α-amylase and glucoamylase, considering particle size, solid:liquid ratio and enzyme load as variables. The hydrolysate, in the best conditions (73 U of α-amylase/g grain and 1150 U glucoamylase/g grain), contained glucose at a concentration of approximately 250 g/L, which was fermented to produce ethanol in a bioreactor in batch mode. Using an industrial strain of Saccharomyces cerevisiae, the maximum ethanol concentration produced was roughly 106 g.L-1 in 24 h of fermentation, resulting in a volumetric productivity of 4.4 g.L-1.h-1 and a product yield based on the substrate consumed (0.499 g.g-1) close to the theoretical.

【 授权许可】

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