Ethanol production from xylose by Pichia stipitis NRRL Y-7124 in a stirred tank bioreactor

Brazilian Journal of Chemical Engineering

J. P. A. Silva²  S. I. Mussatto¹  I. C. Roberto²  J. A. Teixeira¹ 

[1] ,University of São Paulo Engineering College of Lorena Department of BiotechnologyLorena SP ,Brazil

关键词: Ethanol;    Pichia stipitis;    Xylose;    Stirred tank bioreactor;    Volumetric oxygen transfer coefficient;   

DOI  :  10.1590/S0104-66322011000100016

来源: SciELO

【 摘 要 】

The ethanol production by Pichia stipitis was evaluated in a stirred tank bioreactor using semi-defined medium containing xylose (90.0 g/l) as the main carbon source. Experimental assays were performed according to a 2² full factorial design to evaluate the influence of aeration (0.25 to 0.75 vvm) and agitation (150 to 250 rpm) conditions on ethanol production. In the studied range of values, the agitation increase and aeration decrease favored ethanol production, which was maximum (26.7 g/l) using 250 rpm and 0.25 vvm, conditions that gave a volumetric oxygen transfer coefficient (kLa value) of 4.9 h-1. Under these conditions, the ethanol yield factor, ethanol productivity, and the process efficiency were 0.32 g/g, 0.32 g/l.h, and 63%, respectively. These results are promising and contribute to the development of a suitable process for ethanol production from xylose by Pichia stipitis.

【 授权许可】

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