Evaluation of growth, carbazole biodegradation and anthranilic acid production by Pseudomonas stutzeri

Brazilian Journal of Chemical Engineering

A. L. Larentis²  H. C. C. Sampaio²  C. C. Carneiro²  O. B. Martins¹  T. L. M. Alves² 

[1] ,Universidade Federal do Rio de Janeiro Programa de Engenharia Química Laboratório de BioprocessosRio de Janeiro RJ ,Brasil

关键词: Carbazole;    Anthranilic acid;    Biodegradation;    Biodenitrogenation;    BDN;   

DOI  :  10.1590/S0104-66322011000100005

来源: SciELO

【 摘 要 】

The proportion of nitrogenated compounds such as carbazole in heavy fractions of crude oil is higher in Brazil than in other parts of the world. The degradation of this compound by microorganisms has already been described for bacteria such as Pseudomonas stutzeri ATCC 31258. Assays were undertaken to assess the influence of different carbazole concentrations on cell growth, carbazole degradation and the formation of anthranilic acid (an intermediate in the carbazole degradation pathway). The results indicated that there was an accumulation of anthranilic acid in the medium with the higher concentration of substrate (10 g/L), which could be related to the inhibition of Pseudomonas stutzeri growth in an excess of carbazole. With 1 g/L of carbazole, growth was found to be ten times greater (0.37 g dry cell weight/L) and there was no accumulation of anthranilic acid (formation of around 7 mg/L), with complete carbazole degradation after three days.

【 授权许可】

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