Production of biosurfactants from Pseudomonas aeruginosa PA 1 isolated in oil environments

Brazilian Journal of Chemical Engineering

L.m. Santa Anna²  G.v. Sebastian²  E.p. Menezes¹  T.l.m. Alves¹  A.s. Santos¹  N. Pereira Jr.¹  D.m.g. Freire¹ 

[1] ,Federal University of Rio de Janeiro Petrobras Research Center Rio de Janeiro RJ ,Brazil

关键词: production of biosurfactants;    glycolipids;    rhamnolipids;    Pseudomonas aeruginosa;    surface-active substances;   

DOI  :  10.1590/S0104-66322002000200011

来源: SciELO

【 摘 要 】

The potential production of rhamnolipid-type biosurfactants is assessed based on the development of a fermentative process with a strain of Pseudomonas aeruginosa PA1, which was isolated from oil production wastewater in the Northeast of Brazil. These production of molecules using different carbon (n-hexadecane, paraffinic oil, glycerol and babassu oil) and nitrogen sources (NaNO3, (NH4)2SO4 and CH4N2O) was studied. The best results were obtained when using glycerol as substrate. A C/N ratio of 60/1 and use of sodium nitrate as nitrogen source resulted in higher production of the rhamnolipid, expressed by rhamnose (3.16 g/L) and by the yield in relation to biomass (Yp/x = 0.70 g/g). Additionally, physical-chemical characteristics of the spent broth with and without cells were studied, providing a low critical micelle concentration of 19 mg/L and toxicity values of 13 and 13.8 mg/L using two test organisms, the micro crustacean Daphnia similis and the bacterium Vibrio fisheri (Microtox), respectively.

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