Development and characterization of microfiltration hollow-fiber modules for sterilization of fermentation media

Brazilian Journal of Chemical Engineering

L.f.f. Faria²  M. Di Luccio¹  R. Nobrega¹  C.p. Borges¹ 

[1] ,Faculdade de Engenharia Química de Lorena Departamento de Engenharia Química Lorena SP ,Brazil

关键词: sterilization;    membrane processes;    microfiltration;    fermentation;   

DOI  :  10.1590/S0104-66322002000200009

来源: SciELO

【 摘 要 】

Sterilization of fermentation medium involving heat may result in undesirable chemical reactions that alter nutrient concentration and yield products, which interfere in the fermentation performance. Sterilization of heat-sensitive compounds usually involves separate sterilizations of carbon source and nutrient solution. Membrane separation processes are an alternative to thermal processes, as they have many advantages such as the possibility of continuous and modular operation and the use of moderate temperatures. In this context, the objective of this work was the preparation of hollow-fiber membranes and the design of modules suitable for continuous sterilization of fermentation medium. The membrane with the best performance had a maximum pore diameter of 0.2 mu m and a permeability of 42.9 L/m².bar.h for a glucose/peptone/yeast extract medium. A module with 0.26 m² of permeation area was built with these membranes. This module was able to provide a permeate flow rate of 2.2 L/h using a pressure difference of 0.2 bar. The collected permeate was completely sterile, thus confirming the efficiency of this process.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License