Symbiotic efficiency and genetic characteristics of Bradyrhizobium sp. strain UFSM LA 1.3 isolated from Lupinus albescens (H. et Arn)

Scientia Agricola

Marcos Roberto Dobler Stroschein¹  Flávio Luiz Foletto Eltz¹  Zaida Inês Antoniolli¹  Manoeli Lupatini¹  Luciano Kaiser Vargas²  Adriana Giongo²  Mateus Padoin Pontelli¹ 

[1] ,UFSM Depto. de Solos Santa Maria RS ,Brasil

关键词: lupin;    rhizobia;    genotypic approaches;    tremoço;    rizóbio;    análise genética;   

DOI  :  10.1590/S0103-90162010000600012

来源: SciELO

【 摘 要 】

Legume species belonging to the genus Lupinus are annual herb plants. The majority of them are indigenous to the Americas. They are known for nitrogen-fixing symbioses with soil bacteria collectively called rhizobia. The aim of this study was to characterize a rhizobium strain isolated from Lupinus albescens using phenotypic, symbiotic and molecular approaches. Strain UFSM LA 1.3 was tested in vitro according to several parameters: colony size, color and growing rate; acid or alkaline reaction in yeast mannitol media supplemented with bromothymol blue; gum production. Molecular characterization was evaluated by PCR technique using primers BOX A1-R and sequence analysis of the 16S-23S rDNA intergenic region (ITS). ITS sequencing fragments showed genetic similarity with Bradyrhizobium sp. The polymorphism observed by BOX-PCR have shown that strain differs from the reference strain SEMIA 928 and SEMIA 938. The symbiotic efficiency under axenic conditions of UFSM LA 1.3 was 94.6%, without statistical differences compared to the mineral nitrogen fertilized control, to which was applied solution of 400 mg of ammonium nitrate.

【 授权许可】

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