Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Scientia Agricola

Luciana Takada²  Alicio Martins Junior¹  Gisele Zoccal Mingoti¹  Julio César Carvalho Balieiro¹  Lia Alencar Coelho¹ 

[1] ,USP FZEA Programa de Pós-Graduação em Qualidade e Produtividade Animal

关键词: comet assay;    embryos;    gonadotropins;    in vitro fertilization;    melatonin;    ensaio cometa;    embriões;    gonadotrofinas;    fertilização in vitro;    melatonina;   

DOI  :  10.1590/S0103-90162010000400003

来源: SciELO

【 摘 要 】

Melatonin (MEL) acts as a powerful scavenger of free radicals and direct gonadal responses to melatonin have been reported in the literature. Few studies, however, have evaluated the effect of MEL during in vitro maturation (IVM) on bovine embryos. This study tested the addition of MEL to maturation medium (MM) with no gonadotropins on nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos. Cumulus-oocyte complexes were aspirated from abattoir ovaries and cultured in MM (TCM-199 medium supplemented with 10% fetal calf serum - FCS) at 39ºC and 5% CO2 in air. After 24 hours of culture in MM with 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH; 10-9 M MEL) or 10-9 M MEL, 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH, the oocytes were stained with Hoechst 33342 to evaluate nuclear maturation rate. After in vitro fertilization and embryo culture, development rates were evaluated and the blastocysts were assessed for DNA damage by Comet assay. There was no effect of melatonin added to the MM, alone or in combination with gonadotropins, on nuclear maturation, cleavage and blastocyst rates. These rates ranged between 88% to 90%, 85% to 88% and 42% to 46%, respectively. The extent of DNA damage in embryos was also not affected by MEL supplementation during IVM. The addition of 10-9 M MEL to the MM failed to improve nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos, but was able to properly substitute for gonadotropins during IVM.

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