Microsatellite markers for identification of a group of italian olive accessions

Scientia Agricola

Innocenzo Muzzalupo¹  Francesca Stefanizzi¹  Amelia Salimonti¹  Rosanna Falabella¹  Enzo Perri¹ 

[1] ,Consiglio per la Ricerca e la Sperimentazione in Agricoltura Centro di ricerca per l'olivicoltura e l'industria olearia Rende CS ,Italy

关键词: Olea europaea;    fingerprinting;    genetic identity;    molecular markers;    Olea europaea;    fingerprinting;    identidade genética;    marcadores moleculares;   

DOI  :  10.1590/S0103-90162009000500014

来源: SciELO

【 摘 要 】

Cultivar characterization for fruit trees certification requires fast, efficient and reliable techniques. Microsatellite markers (SSR) were used in the molecular characterization of 23 genotypes of Olea europaea subsp europaea. The DNA from the olive cultivars was analyzed using nine pre-selected SSR primers (GAPU59, GAPU71A, GAPU71B, GAPU103A, UDO99-01, UDO99-12, UDO99-28 and UDO99-39) and revealed 29 alleles, which allowed each genotype to be identified. In the dendrogram, the nine primers allowed the 23 olive genotypes to be grouped into subgroups corresponding to the same cultivar denominations. SSR markers proved to be efficient and reliable for the molecular characterization of Italian olive cultivars.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License