Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost

Scientia Agricola

Chen-chin Chang²  Chang-chai Ng¹  Chung-yi Wang¹  Yuan-tay Shyu¹ 

[1] ,Tainan University of Technology Dept. of Home Science Yongkang Tainan ,Taiwan ROC

关键词: cellulose degrading;    enzymes;    vegetable wastes;    degradação de celulose;    enzimas;    resíduos vegetais;   

DOI  :  10.1590/S0103-90162009000300004

来源: SciELO

【 摘 要 】

Plant wastes present a high cellulose content, which is an ideal organic material for composting. Five strains of thermophiles from processed Brassica waste were isolated, and the hydrolytic activity on various cellulosic biomass substrata and their temperature profiles were determined. 16S rRNA sequencing identified these strains as Thermoactinomyces and Bacillus spp. Maximal cellulase activity corresponded to 2.3 U mL-1 of enzyme. The application of these strains on Brassica rapa residues demonstrates increased total nitrogen content). TA-3, a Thermoactinomycetes sp. strain, performs best among all inoculants, increasing the nitrogen content from 0.74 to 0.91%, and decreasing the carbon content from 15.4 to 12.2%, showing its high efficiency and bioactivity during compositing.

【 授权许可】

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