Chlorotic spots on Clerodendrum, a disease caused by a nuclear type of Brevipalpus (Acari:Tenuipalpidae) transmitted virus

Scientia Agricola

Elliot Watanabe Kitajima²  Karen Sumire Kubo¹  Paulo De Tarso Oliveira Ferreira¹  Berenice Kussumoto De Alcântara¹  Alessandra Jesus Boari¹  Renata Takassugi Gomes¹  Juliana Freitas-astua¹  Jorge Alberto Marques Rezende²  Gilberto José De Morais²  Renato Barbosa Salaroli² 

[1] ,USP ESALQ Depto. de Entomologia, Fitopatologia e Zoologia AgrícolaPiracicaba SP ,Brasil

关键词: Brevipalpus phoenicis;    Clerodendrum x speciosum;    host range;    transmission;    purification;    Breviplapus phoenicis;    Clerodendrum x speciosum;    transmissão;    gama de hospedeiras;    purificação;   

DOI  :  10.1590/S0103-90162008000100006

来源: SciELO

【 摘 要 】

Chlorotic spots have been observed in plants of Clerodendrum x speciosum growing in residential gardens and parks in Piracicaba, SP, Brazil. Thin sections of diseased tissues revealed characteristic cytopathic effects of the nuclear type of the Brevipalpus (Acari: Tenuipalpidae) mite-transmitted viruses (BTrV). Brevipalpus mites, identified as B. phoenicis, infesting symptomatic C. x speciosum plants transmitted the pathogen to healthy C. x speciosum and to C. thomsonae, Gomphrena globosa, Hibiscus cannabinus, H. coccineus, H. schizopetalus, Salvia leucantha, Spathiphyllum wallasi and Tetragonia expansa causing chlorotic spots on their leaves. Mechanical inoculation using leaf extracts from infected C. x speciosum resulted in chlorotic spots on inoculated C. x speciosum, Chenopodium quinoa, C. amaranticolor, G. globosa, H. cannabinus, H. coccineus and T. expansa leaves. C. amaranticolor and C. quinoa kept at 28 - 30°C became systemically infected. The same cytopathic effects caused by the nuclear type of BTrV were seen in tissues from all infected test plants by electron microscopy. The virus was purified from systemically infected leaves of C. amaranticolor and C. quinoa. A polyclonal antiserum obtained from an immunized rabbit presented a strong reaction with the homologous antigen in ELISA tests. The results suggest that this chlorotic spot disease of C. x speciosum is caused by a new species of the nuclear type of BTrV, tentatively named Clerodendrum chlorotic spot virus (ClCSV).

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