Determination of aspartate kinase activity in maize tissues

Scientia Agricola

Renato Rodrigues Ferreira²  Ariane Vendemiatti¹  Priscila Lupino Gratão¹  Peter John Lea¹  Ricardo Antunes Azevedo¹ 

[1] ,UNICAMP IB Depto. de Genética e EvoluçãoCampinas SP ,Brasil

关键词: aspartate pathway;    maize;    lysine;    threonine;    methionine;    via do aspartato;    milho;    lisina;    treonina;    metionina;   

DOI  :  10.1590/S0103-90162005000200015

来源: SciELO

【 摘 要 】

Lysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity.

【 授权许可】

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