Molecular identification of Aspergillus spp. isolated from coffee beans

Scientia Agricola

Marciane Magnani¹  Thiago Fernandes²  Cássio Egidio Cavenaghi Prete²  Martin Homechim²  Elisabete Yurie Sataque Ono¹  Laurival Antonio Vilas-boas²  Daniele Sartori²  Márcia Cristina Furlaneto²  Maria Helena Pelegrinelli Fungaro² 

[1] ,Universidade Estadual de Londrina Departamento de Bioquímica Londrina PR ,Brasil

关键词: ITS;    ribosomal DNA;    ochratoxin;    mycotoxin;    ITS;    DNA ribossomal;    ocratoxina;    micotoxina;   

DOI  :  10.1590/S0103-90162005000100009

来源: SciELO

【 摘 要 】

Some species belonging to the genus Aspergillus are potential producers of ochratoxin A (OA), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. The aim of the present study was to identify the species of Aspergillus that contaminate the inside of coffee beans collected in the stage of maturation and drying, from 16 producing areas located in the northern region of the State of Paraná, in the South of Brazil. A total of 108 isolates of Aspergillus spp. was identified at the species level, by sequencing the internal transcribed spacer (ITS1-5.8S-ITS2) of ribosomal DNA (rDNA). The results revealed the presence of potentially ochratoxigenic species in 82% of the geographic regions studied, among which Aspergillus niger was the species most frequently detected, followed by A. ochraceus and A. carbonarius. The presence of A. carbonarius in immature coffee fruits harvested from trees is reported for the first time.

【 授权许可】

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