Nitrogen-15 labeling of Crotalaria juncea green manure

Scientia Agricola

Edmilson José Ambrosano²  Paulo Cesar Ocheuze Trivelin¹  Heitor Cantarella¹  Raffaella Rossetto²  Takashi Muraoka¹  José Albertino Bendassolli¹  Gláucia Maria Bovi Ambrosano¹  Luciano Grassi Tamiso¹  Felipe De Campos Vieira¹  Ithamar Prada Neto¹ 

[1] ,IAC Estação Experimental de Agronomia de Piracicaba Centro de Ação RegionalPiracicaba SP

关键词: 15N -fertilizer;    labeled legumes;    green manure;    fertilizante-15N;    leguminosa marcada;    adubo verde;   

DOI  :  10.1590/S0103-90162003000100027

来源: SciELO

【 摘 要 】

Most studies dealing with the utilization of 15N labeled plant material do not present details about the labeling technique. This is especially relevant for legume species since biological nitrogen fixation difficults plant enrichment. A technique was developed for labeling leguminous plant tissue with 15N to obtain labeled material for nitrogen dynamics studies. Sun hemp (Crotalaria juncea L.) was grown on a Paleudalf, under field conditions. An amount of 58.32 g of urea with 70.57 ± 0.04 atom % 15N was sprayed three times on plants grown on eight 6-m²-plots. The labelled material presented 2.412 atom % 15N in a total dry matter equivalent to 9 Mg ha-1 This degree of enrichment enables the use of the green manure in pot or field experiments requiring 15N-labeled material.

【 授权许可】

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