Calcium, potassium and magnesium treatment of Chrysanthemum morifolium cv. "Bi Time" and callogenesis in vitro

Scientia Agricola

Fábio Borgatto²  Carlos Tadeu Dos Santos Dias¹  Antônio Francisco Campos Amaral²  Murilo Melo² 

[1],USP ESALQ Depto. de Ciências BiológicasPiracicaba SP

关键词: matrix plant;    mineral nutrition;    tissue culture;    planta matriz;    nutrição mineral;    cultura de tecido;   

DOI  :  10.1590/S0103-90162002000400011

来源: SciELO

【 摘 要 】

The chemical composition and vegetative vigor of the donor plant are essential for the satisfactory performance of explants in vitro. In order to test the effect of potassium, calcium and magnesium nutritional status of Chrysanthemum morifolium plants on callogenesis in vitro, pot plants growing in sand were irrigated with nutrient solution containing different levels of potassium (0; 58.5; 117 and 234 mg L-1), calcium (0; 50; 100 e 200 mg L-1) and magnesium (0;12; 48 e 96 mg L-1). After 30 and 45 days, explants (shoot segments and leaf discs) were collected, desinfected and inoculated on MS solid medium supplemented with 0.1 mg L-1 of kinetin and 5.0 mg L-1 of nafthalene acetic acid for callogenesis induction. Callogenesis evaluated as callus fresh weight was affected by nutrients treatment. Callus growth on leaf explants was inversely proporcional to potassium concentration and directly proportional to magnesium concentration in shoot explants. The calcium effect on callogensis of leaf explants was dependent on treatment duration. For 30 days treatment callogenseis was inversely related to calcium concentration and after 45 days was directly related to calcium concentration.

【 授权许可】

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