Growth of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. cell suspension cultures with carbon sources

Scientia Agricola

Marcia Ometto Mello²  Carlos Tadeu Santos Dias¹  Antônio Francisco Campos Amaral²  Murilo Melo² 

[1] ,USP ESALQ Depto. de Ciências BiológicasPiracicaba SP

关键词: sucrose;    glycerol;    sorbitol;    galactose;    tissue culture;    sacarose;    glicerol;    sorbitol;    galactose;    cultura de tecido;   

DOI  :  10.1590/S0103-90162001000300007

来源: SciELO

【 摘 要 】

Cell suspension cultures of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. were used to test plant ability to utilize an alternative to sucrose as carbon source and energy for growth. Glycerol, sorbitol and galactose were the alternative carbon sources tested. Cell suspension cultures established on liquid medium containing sucrose were transferred to culture medium supplemented with sucrose or glycerol, or sorbitol, or galactose as the sole carbon source. Fresh and dry weight increasing and protein content showed marked differences among the different carbon sources used. Sucrose was the best carbon source for all the three plant species tested. Galactose and glycerol promoted slow or no growth of the three studied species. Sucrose in liquid medium promoted initiation of meristemoid formation. Sorbitol, which was ineffective on promoting significant growth, was the only alternative carbon source tested that also promoted this effect.

【 授权许可】

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