Molecular characterization of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) isolates

Scientia Agricola

Andréia Cristiane Souza Azevedo²  Márcia Cristina Furlaneto¹  Daniel Ricardo Sosa-gómez¹  Maria Helena Pelegrinelli Fungaro² 

[1] ,UEL CCB Depto. de Biologia GeralLondrina PR

关键词: Paecilomyces fumosoroseus;    Bemisia;    RAPD;    biological control;    entomopathogenic fungus;    Paecilomyces fumosoroseus;    Bemisia;    RAPD;    controle biológico;    fungo entomopatogênico;   

DOI  :  10.1590/S0103-90162000000400021

来源: SciELO

【 摘 要 】

ITS and RAPD analyses were used to investigate molecular variations within samples of Paecilomyces isolates and to resolve five morphologically atypical isolates resembling P. fumosorosus, obtained from whitefly in Northern Paraná State. The ITS4-ITS5 amplicon was 700 base pairs (bp) long in all isolates. The five isolates of Paecilomyces not assigned to species produced restriction profiles identical to all the reference strains of P. fumosoroseus. The extent of fingerprint variability observed by RAPD was sufficient to discriminate all the isolates. The genetic similarity among unidentified isolates and strains of P. fumosoroseus was even higher than that observed among reference strains of this species, allowing us to conclude that isolates CNPso-P77, CNPso-P78, CNPso-P80, CNPso-P85 and CNPso-P91 are P. fumosoroseus.

【 授权许可】

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