Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite

Ciência Rural

Elisângela França Soares¹  Anna Carolina Da Silva¹  Alana Emilia Soares De França Queiroz¹  José Erick Galindo Gomes¹  Polyanna Nunes Herculano¹  Keila Aparecida Moreira¹ 

关键词: enzimas coagulantes do leite;    atividade proteolítica;    coagulante vegetal;    fruta-pão;    milk-clotting enzyme;    proteolytic activity;    plant rennet;    breadfruit;   

DOI  :  10.1590/0103-8478cr20140310

来源: SciELO

【 摘 要 】

Breadfruit is an exotic tree in Brazil, very well acclimatized. Despite of being a laticifer plant, the knowledge about its latex is scarce. However, it is known that proteolytic enzymes represents over 50% of the latex composition in laticifers plants. The aim of this study was to evaluate the potential of breadfruit (Artocarpus altilis var. Apyrena) latex as a source of milk clotting proteases and partially characterize it. The proteolytic activity of the fraction of crude extract was assessed using azocasein and quantitation of total protein, the bicinchoninic acid (BCA). Milk-clotting activity was analyzed using skim milk at 12%. The enzyme activity was analyzed under different temperature conditions (35 to 80°C) and pH (5.8 to 10.7) presenting optimum activity in alkaline pH (8.5) and 50°C; being stable to the two variables at 120 minutes during the test. The clotting activity was directly proportional to the temperature at the better concentration of CaCl2 (10mmol L-1). The results indicate that enzyme is a possible replacement for calf rennet

【 授权许可】

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